Hippuric acid in urine: reference values
نویسندگان
چکیده
منابع مشابه
Colorimetric determination of hippuric acid in urine and liver homogenate.
To a dried extract Containing hippuric acid (HA), 1.0 mL of acetic anhydride and 2.0 mL of 0.5% p-dlmethyiamlnobenzaldehyde (DAB) solution In pyridine were added, and the solution was kept at 40 O C for 1 h after thorough mlxing. The absorbance was then determlned at 458 nm agalnst a blank containing acetic anhydride, DAB, and pyridine. This method was in good agreement with Beer’s law within 1...
متن کاملDirect colorimetric determination of hippuric acid in urine.
In 1963 Umberger and Fiorese (1) introduced a colorimetric method for HA,1 based on the color reaction of HA, dissolved in anhydrous pyridine, with BSC. Therefore, for applying their method to determine HA in urine, HA must first be extracted from the urine. We found that HA dissolved in water-pyridine (1:1, by vol) produces a red-orange color when BSC is added at room temperature. The absorpti...
متن کاملHippuric acid analysis of urine using the Hofmann rearrangement.
1. METHOD FOR THE determination of hippuric acid based on the formatioll of a bromine derivative was deScrii)ed by Deniges (1). This bromine derivative has been determined in our laboratory to be Nbromobenzamide. N-bromobenzamide is the hydrolysis and bromination product of hippuric acid, being produced when hippuric acid is subjected to the Hofniann rearrangement, tile first step of which is a...
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We describe a project, participated in by 24 institutions in The Netherlands and Belgium, to determine normal reference values for steroids in urine by capillary gas chromatography. Urine samples from 288 healthy volunteers were analyzed in triplicate. Reference values, expressed in mumol/24 h, were determined for androsterone, etiocholanolone, dehydroepiandrosterone, 11-keto-androsterone, 11-k...
متن کاملReliability of Urinary Excretion Rate Adjustment in Measurements of Hippuric Acid in Urine
The urinary excretion rate is calculated based on short-term, defined time sample collections with a known sample mass, and this measurement can be used to remove the variability in urine concentrations due to urine dilution. Adjustment to the urinary excretion rate of hippuric acid was evaluated in 31 healthy volunteers (14 males and 17 females). Urine was collected as short-term or spot sampl...
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ژورنال
عنوان ژورنال: Revista de Saúde Pública
سال: 2002
ISSN: 0034-8910
DOI: 10.1590/s0034-89102002000700010